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Western blotting For western blotting experiments, tissues were lysed with Magnalyser beads in radioimmuno-precipitation assay (RIPA) buffer (50 mM Tris-HCl, 150 mM NaCl, 2 mM EDTA, 50 mM NaF, 0.1% SDS, 0.5% Nadeoxycholate, 1% NP-40, adjusted to pH 7.27.4) supplemented with PIC (complete Tablets EASYpack, Roche, Basel, Switzerland) and PhosStop (Roche, Basel, Switzerland)