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The unbound fraction was released and next the resin was washed with: 5 litres of sodium phosphate buffer (50 mM Na 2 HPO 4 , 300 mM NaCl, pH 7.5) for glutathione Sepharose or in the case of His-tag modification the phosphate buffer (50 mM Na 2 HPO 4 , 300 mM NaCl, pH 7.5) was enriched with imidazole 500 mM

AOD-9604 is primarily used in research focusing on metabolic health, particularly its potential for targeted fat reduction (lipolysis) and inhibition of fat creation (lipogenesis)

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However, incorporating a complex microbiota-on-chip presents challenges like simulating the anaerobic-to-aerobic gradient, maintaining high microbial diversity and using oxygen gradients to culture both anaerobes and aerobes simultaneously