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RNA extraction, quantification of miRNA expression and qPCR Total RNA was extracted from the lung tissue samples of the screening cohort and from PBECs derived from non-COPD and COPD patients by TRIzol reagent method (Molecular Research Center, Cincinnati, OH) according to manufacturer's guidelines

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Whether you are using vaping as a smoking cessation tool or for recreational purposes, its important to manage your usage to avoid potential health risks and dependency