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Immune cell isolation from induced sputum and peripheral blood To eliminate mucus from sputum samples, the same volume of 0.1% dithiothreitol (Sigma, MO, USA) was added, the tube was shaken for 20 min at 37 C, and the mixture was filtered through a 70 m strainer

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Too bad, fire is prevented but there is no cleaning up what comes in the machinery, pure filth just waiting to make it nerve-racking