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The results showed that in the nicotine treatment group, expression of the virulence-related genes hla , hlb , pvl , nuc , ssp , spa , and sigB was downregulated at both 12 h and 24 h, the expression of rsbU decreased at 12 h, whereas only the coagulase gene coa and the pigmentation-related gene crtN was downregulated at 24 h (Fig
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