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In Vivo Subchronic Inhalation Testing The in vivo animal toxicology portion of Project MIX consisted of exposing male and female Sprague-Dawley rats, nose only, to either fresh air (sham) or diluted mainstream smoke from the Project MIX control and test cigarettes for 90 d, followed by a 42-d postexposure period, after which time the animals were sacrificed and histopathology studies were completed [18]

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Patients were divided into three groups: (1) non-smoking patients, (2) patients with consumption of classic cigarettes (with or without additional consumption of e-cigarettes and/or hookahs), and (3) patients with exclusive consumption of e-cigarettes and/or hookahs (without additional consumption of classic cigarettes)