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qPCR was carried out in 384-well plates on the ABI PRISM 7900HT Sequence Detection System (Applied Biosystems)

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Accordingly, CMS would only permit ACOs that are currently participating in the Shared Savings Program, that have earned shared savings in the most recent performance year for which financial reconciliation has been completed, and that have a positive prior savings adjustment at application disposition to receive prepaid shared savings, as they have the history of success that provides CMS with a more reasoned expectation that they will earn shared savings in the future

(c) Timing