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Back in the day, rolling a joint was an art form, and often a rite of passage, requiring time, skill, and a steady hand

Briefly, cigarette smoke from one cigarette was collected from the side of the combustion zone with neither puffing nor passing through a filter, and bubbled into 10 mL of sterile PBS in a 50 mL centrifuge tube at minimum speed using a suction pump (Tokyo M.I., Tokyo, Japan, Cat# M20)

At least 3/32 inch thick for tubes 18 to 32 inches long

American Spirit tobacco products might be common enough in other parts of the world, but Japan is currently the only country in Asia where they can be purchased, which is probably why American Spirit cigarettes tend to be top sellers in Japanese airport duty-free shops