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The teen has since undergone numerous surgeries and suffers permanent facial damage
Purified gDNA was quantified by Qubit BR and 100 ng of gDNA was tagmented to an average length of 600 bp with a Tn5 transposome, purified from pTXB1-Tn5 (Addgene, 60240), assembled with oligos containing a 10-nt unique molecular identifier and then purified using solid-phase reverse immobilization (SPRI) guanidine magnetic beads
We showed that in mice genetically engineered to lack secretory IgA, moDCs activate T lymphocytes white blood cells that fight off viruses and can destroy cells in the process and those T lymphocytes in turn damage the lungs
J Health Sci 2009, 55: 259264
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